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Title: 15N-CPMAS nuclear magnetic resonace spectroscopy and biological stability of soil organic nitrogen in whole soil and particle-size fractions

Author: DiCosty, R.J.; Weliky, D.P.; Anderson, S.J.; Paul, E.A.;

Date: 2003

Source: Organic Geochemistry 34 (2003) 1635-1650

Publication Series: Miscellaneous Publication

Description: Soil organic nitrogen was quantified by solid-state 15N cross-polarization nuclear magnetic resonance spectroscopy (NMR) during a 14-month laboratory incubation of a sandy loam soil amended with 15N-clover. In whole soil and particle-size fractions, the clover-derived N was always 85-90% amide, 5 10% guanidinium N of arginine, and 5% amino. Quantitativeness of these results was suggested by (1) analysis of a standard containing a complex mixture of organic 15N and (2) correlation of spectral intensities with 15N concentrations. Based on the unchanging proteinaceous NMR signature of clover-derived N throughout the incubation, differences in the mineralization/immobilization kinetics of clover-N among the different particle-size fractions appeared not to be linked to organic functional group. Kinetic analysis of the mineralization of 15N, with correction of rate constants for field temperatures, suggested that the proteinanceous 15N in the clay and fine silt fractions observed here had a mean residence time of 7 years in the field.

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DiCosty, R.J.; Weliky, D.P.; Anderson, S.J.; Paul, E.A. 2003. 15N-CPMAS nuclear magnetic resonace spectroscopy and biological stability of soil organic nitrogen in whole soil and particle-size fractions. Organic Geochemistry 34 (2003) 1635-1650

 


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