Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (249 KB bytes)

Title: Growth and fermentation responses of Phanerochaete chrysosporium to O2 limitation

Author: Kenealy, William R.; Dietrich, Diane M.;

Date: 2004

Source: Enzyme and microbial technology. Vol. 34 (2004): Pages 490-498.

Publication Series: Miscellaneous Publication

Description: Phanerochaete chrysosporium BKM-F-1767 produced small amounts of ethanol from glucose, mannose, cellobiose, maltose and sucrose when grown with a limited O2 supply in sealed bottles. Under O 2 -limited growth on glucose, low levels of acetate or oxalate were produced when nitrogen was in excess or limited, respectively. Alcohol dehydrogenase activity (15 nmol/min/mg extract protein) was detected in cell extracts made from ethanol producing cells. The low levels of fermentative enzymes and products indicate that P. chrysosporium does not grow fermentatively, but probably survives transient oxygen limitation by fermentation. Analysis of the genome sequence has indicated multiple alcohol dehydrogenase genes that resemble ADH I and ADH II. Fermentation experiments conducted with 1-13 C-and 2-13 C-glucose produced 2-13 C- and 1- 13 C-ethanol, respectively. Under conditions of 1- 13 C-glucose incubation with nitrogen in excess 2-13 C- acetate was also detected. The labeling pattern is consistent with ethanol formation by glycolysis and alcohol dehydrogenase. Ethanol was detected in aspen chips aerobically colonized by P. chrysosporium only when the culture was purged with nitrogen gas. The data indicate that P. chrysosporium is able to ferment components of wood when the supply of 02 is depleted.

Keywords: Phanerochaete chrysosporium, fermentation, wood, ethanol, oxalate, white-rot fungi

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Kenealy, William R.; Dietrich, Diane M. 2004. Growth and fermentation responses of Phanerochaete chrysosporium to O2 limitation. Enzyme and microbial technology. Vol. 34 (2004): Pages 490-498.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.